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. 2013 May 22;8(5):e64829. doi: 10.1371/journal.pone.0064829

Figure 6. hCG modulates the expression of immune, angiogenic and tissue remodeling factors in ESCs at the protein level.

Figure 6

Confluent ESC cultures were incubated with minimal medium (MM) or hCG (100 ng/mL) for 24 h before being exposed or not to IL1B (0.1 ng/mL) for additional 24 h. Supernatant was collected and soluble proteins CCL2 (A) CCL5 (B) VEGFC (C) TIMP3 (D) MMP9 (E) were then quantified by ELISA. Data were from ESC cultures issued from 4 different subjects and expressed in pg/mL. *P<0.05, *** P<0.001 relative to MM; ††P<0.01, †††P<0.001 relative to cells stimulated with an equivalent concentration of hCG; +P<0.05, +++P<0.001 relative to cells stimulated with an equivalent concentration of IL1B.