Figure 6. Analyzing Mj-FAR-1 functions in manipulating plant defense pathways.

Expression level of 11 defence-related target genes in mj-far-1 expressing root line far-1 E1.1 compared with vector control prior and 5 DAI with Meloidogyne javanica. Total RNA was prepared from Vector 11.5 transformed control roots and roots expressing mj-far-1 with/without infection. The graph shows the mean and standard error of the relative amount of transcripts of these genes in mj-far-1 expressing roots (far-1 E1.1) in comparison with vector transformed control roots (Vector 11.5) growing under the same conditions (vector control expression level set at zero). All target genes were normalized using the normalisation factor calculated as the geometric mean of the expression levels of three tomato endogenous reference genes 18S, actin and β-tubulin. Each reaction was performed in triplicate and the results represented the mean of two independent biological replicates. Statistical significance of the differences between far-1 E1.1 and Vector 11.5 transformed control roots were determined by Tukey-Kramer multiple comparison test, and significant differential expression (P≤0.05) is indicated with asterisks. The experiments were repeated three times with similar results.