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. 2013 May 22;8(5):e61202. doi: 10.1371/journal.pone.0061202

Figure 5. Transfer of labile iron from NAF-1 to mitochondria.

Figure 5

Upper panel. Pseudo-colored images of permeabilized h9c2 cells labeled with red rhodamine B-[(1,10-phenanthrolin-5-yl)] benzyl ester (RPA) to trace iron in the mitochondrial matrix and then measured for fluorescence every two minutes. NAF-1 (WT or H114C mutated) was added to either 0, 5, 10, or 20 μM concentrations after 4 minutes (only 20 μM profiles are shown). The pseudocolor of the cells indicates the relative levels of mitochondrial RPA fluorescence (orange: high; blue: low). The liposoluble permeant FHQ, was added to 5 μM after 22 min in order to attain maximum quenching. Lower. Plot of RPA fluorescence is given in terms of arbitrary units (a.u.) obtained by analyzing individual cell fluorescence with Image J as described in Experimental Procedures. The RPA fluorescence is the average of four independent runs.