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TECs were IFN-γ/TNF-α stimulated for 24, 48 and 72 hours. CCL20 mRNA expression by TECs was analyzed by PCR and the supernatant was analyzed for CCL20 protein using ELISA. The dotted line represents the lowest CCL20 detection limit (7.8 pg/mL). No significant CCL20 production could be detected after IFN-γ/TNF-α stimulation (n = 8).
