Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 May 22;8(5):e64916. doi: 10.1371/journal.pone.0064916

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Demmers et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Differential effects of renal tubular epithelial cells on T-cell migration. — (A) PBMCs were activated with anti-CD3/CD28, IL-2 and IFN-γ and placed in a 3.0 µm pore transwell system upon unstimulated TECs or 24 hours IFN-γ/TNF-α stimulated TECs. Activated CD4⁺ T cells expressed 76% CXCR3 and 68% CCR6. (B) Significant migration of CD4+CXCR3+ T cells, containing Th1 cell pool, by activated TECs was observed (P<0.05). No migration of CD4+CCR6, containing Th17 cell pool, could be detected. CD7⁺ and CD16^-/CD56^- lymphocytes were discriminated as anti-CD3/CD28 activated T lymphocytes. (C and D) Transwell migration results of 9 individual experiments using unstimulated TECs vs. stimulated TECs are shown. The total cell number of migrated CD4⁺CXCR3⁺ and CD4⁺CCR6⁺ T cells are depicted on the Y –axis. Results were obtained by flow cytometry using fixed time point and fixed volume. * Significant increase (P <0.05).