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. 2013 May 22;8(5):e64633. doi: 10.1371/journal.pone.0064633

Figure 2. The effect of glucokinase activator on the expression of the incretin receptors under a low glucose concentration.

Figure 2

A), B). Isolated islets were incubated with DMSO (ctl) or GKA (G: 30 µM compound A) in the presence of 2.8 mM glucose for 24 hours. Total cell extracts from the isolated islets were subjected to immunoblotting for p-AMPKα (Thr172) and AMPKα. Data shown are representative of three independent experiments. B) The GLP-1 and GIP receptor expressions were determined by real-time quantitative RT-PCR and normalized to the β actin mRNA level and to the control samples (n = 4). *P<0.05, ** P<0.01.