Figure 4. The impact of manipulation of AMPK phosphorylation by DN-AMPK expression on the expressions of the incretin receptors.

A), B) Isolated islet cells in monolayer were infected with the Ad-AMPK-α2-K45R or AdCMV-LacZ virus in the presence of 2.8 mM glucose for 24 h. A) Total islet extracts from the uninfected cells and virus-infected cells were subjected to immunoblotting for p-AMPKα (Thr¹⁷²) and AMPKα. Data shown are representative of three independent experiments. B) The GLP-1 and GIP receptor expressions were determined by real-time quantitative RT-PCR and normalized to the β actin mRNA level and to the control samples (n = 3). The samples are from independent experiments. *P<0.05 and ** P<0.01.