Figure 5. The effect of glucokinase activator on the expression of the incretin receptors under a medium glucose concentration.

A), B). Isolated islets were incubated with DMSO (ctl) or GKA (G: 30 µM compound A) in the presence of 11.1 mM glucose for 24 hours. Total cell extracts from the isolated islets were subjected to immunoblotting for p-AMPKα (Thr¹⁷²) and AMPKα. Data shown are representative of three independent experiments. B) The GLP-1 and GIP receptor expressions were determined by real-time quantitative RT-PCR and normalized to the β actin mRNA level and to the control samples (n = 4). *P<0.05, ** P<0.01.