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. 2013 May 22;8(5):e63643. doi: 10.1371/journal.pone.0063643

Figure 2. Representative image of synaptophysin-immunoreactive presynaptic boutons (SIPBs) in the hippocampus of a 12-month-old wild-type mouse.

Figure 2

A: In the hippocampus SIPBs were analyzed in the inner (yellow) and outer (red) molecular layer of the dentate gyrus, stratum radiatum (SR) of the CA1 area (blue), and stratum lucidum (SL) of the CA3 area (green). Scale bar = 200 µm. B: SIPBs were quantified with an 100× objective using image analysis from digitized photomicrographs of the synaptophysin-immunoreactivity. Scale bar = 10 µm.