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. 2013 May 17;62(6):1913–1922. doi: 10.2337/db12-0325

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© 2013 by the American Diabetes Association.

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FIG. 2. — The effect of VASP on lipid metabolism in AML12 cells. A: AML12 cells were transduced with VASP (VASP-OE) or control (ctl) (empty) vector. B: RT-PCR analysis of lipid metabolism–related genes in AML12 cells. Expression of Gapdh as shown by the ratio of CT value (n = 3). C: Rate of [1-¹⁴C]palmitate incorporation into acid-soluble metabolites (n = 4). D: Oleic acid (either 0.1 or 0.4 mmol/L)-induced accumulation of TG in AML12 cells. BSA (1.3 mmol/L) was used as a control (n = 3). *P < 0.05. CT, threshold cycle. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IB, immunoblot.