FIG. 3.

AMPK signaling is regulated by VASP in the liver. A: Phosphorylation of AMPK (Thr172) and ACC (Ser79) in AML12 cells with VASP overexpression. AICAR was used as a positive control. Western blot from one of three independent experiments is shown. B: Primary hepatocytes were isolated from WT or Vasp^−/− mice and cultured. p-AMPK (Thr172) and p-ACC (Ser79) as measured by Western blot. Representative blots are shown. Rate of [1-¹⁴C]palmitate incorporation into acid-soluble metabolites (n = 3). C: p-AMPK and p-ACC in the liver collected after an overnight fast (n = 6). Representative blots are shown. *P < 0.05. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IB, immunoblot.