FIG. 3.

Increased frequencies of adipose tissue F4/80⁺CD11c⁺CD206⁺ macrophages in obese LGALS3^−/− mice correlate with body weights and blood glucose levels in LGALS3^−/− mice. A: LGALS3^−/− mice fed HFD for 11 weeks have significantly increased percentages of F4/80⁺CD11c⁺CD206⁺ macrophages in VAT compared with other experimental groups. B: LGALS3^−/− fed HFD have significantly lower percentages of alternatively activated F4/80⁺CD11c-CD206⁺ M2 macrophages in VAT compared with WT mice fed LFD. C: Positive correlation between the frequencies of proinflammatory F4/80⁺CD11c⁺CD206⁺ macrophages with body weights (r = 0.919, P = 0.001) and fasting blood glucose levels (r = 0.912, P = 0.002) in LGALS3^−/− mice. D: Inverse correlation between the frequencies of Tregs with body weights (r = 0.633, P = 0.067) and blood glucose levels (r = 0.819, P = 0.007) in LGALS3^−/− mice. r, Pearson correlation coefficient. The results are shown as the means ± SEM for four to six animals per group. SVF, stromal vascular fraction. *P < 0.05; §P < 0.05.