FIG. 1.
A: Rac1-GTP binding and p-PAK1Thr423 in isolated incubated mouse soleus and EDL muscle (n = 8). B: Rac1-GTP loading and p-PAK1Thr423 in human VL muscle prior to and after a 2-h hyperinsulinemic-euglycemic clamp (n = 8). C: Representative images of Rac1 localization in mouse EDL single fibers ± 10 min 60 nmol/L ex vivo insulin stimulation and bar graph showing mean ± SEM. Arrows indicate magnification of the highlighted area in the top panel. Full width at half maximum (FWHM) of the striated Rac1 staining (n = 4). D: Bar graphs showing protein expression of PAK1 in human (n = 8) and mouse (n = 24) skeletal muscle of different fiber type composition. Statistical significance between basal and insulin or between muscles: *P < 0.05; **P < 0.01. Values are mean ± SEM. A.U., arbitrary units.