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. 2013 May 17;62(6):1888–1896. doi: 10.2337/db12-1502

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© 2013 by the American Diabetes Association.

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FIG. 5. — Chronic PEGPH20 treatment decreases adipocyte size and gene expression of the inflammatory markers. A: Immunochemical detection of HA and Toluidine Blue O staining in the perigonadal adipose tissue of vehicle and chronic PEGPH20-treated DIO mice. B: Adipocyte size was determined by manual measurements of adipocyte diameters of at least 100 adipocytes per mouse. C: Gene expression in perigonadal adipose tissue. Data were normalized to 18S expression. Mθ, macrophage; M1, proinflammatory markers; M2, anti-inflammatory markers. D: Liver triglyceride content in vehicle- and chronic PEGPH20-treated DIO mice. n = 6–8. *P < 0.05 vs. vehicle. All data are represented as mean ± SEM. (A high-quality color representation of this figure is available in the online issue.)