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. 2013 May 20;62(6):1945–1956. doi: 10.2337/db12-0828

FIG. 2.

FIG. 2.

Adipose tissue reduction of 17-HDHA and PD1 represents one of the earliest alterations in diet-induced inflammation. WT mice were fed a normal standard chow (NC; □) or an HF diet (■) for 4 or 14 days. (A) mRNA expression of the genes for macrophage marker F4/80 (encoded by Emr1 gene) and MCP-1 (encoded by Ccl2) was determined in gonadal adipose tissue using quantitative real-time RT-PCR (n = 12 animals per group). (B) Mean body weight after the NC or HF diet for 4 and 14 days (n = 12–15 animals per group). (C–F) Lipid mediator levels were analyzed in gonadal adipose tissue. PGE2 was increased after 4 days but was significantly lower after 14 days of the HF diet. The HF diet decreased adipose tissue levels of 12/15-LOX–derived 17-HDHA and PD1 after 4 days and demonstrated an even stronger impact after 14 days (n = 12–15 animals per group). All data are mean ± SEM. #P = 0.088; *P < 0.05; **P < 0.01; ***P < 0.001.