FIG. 2.

No significant difference was found in food consumption or lipid metabolism between apelin KO and WT mice after HFD. A: Food intake was similar in apelin KO mice and WT littermates. B: Obese apelin KO mice had a lower plasma insulin level, but the difference was not significant. C: Serum leptin levels were approximately two times higher in apelin KO mice than in WT mice (**P < 0.01). D: Serum adiponectin levels tended to be lower in apelin KO mice, but the difference was not significant. There was no significant difference of plasma glucose (E), HDL cholesterol (HDLC; F), triglyceride (G), or free fatty acid (FFA; H) between apelin KO mice and WT mice. Data are expressed as mean values ± SD (N = 7; **P < 0.01, †P < 0.1). N.S., not significant.