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. 2013 May 20;62(6):1970–1980. doi: 10.2337/db12-0604

FIG. 6.

FIG. 6.

Resistance to obesity in K14-apelin mice. A: Change in body weight of HFD-fed K14-apelin and WT mice (N = 4–7). B: Average weekly food intake of each genotype. C: Hematoxylin and eosin staining of skin sections from HFD-fed, K14-apelin, and WT mice. A reduced subcutaneous adipose layer was found in K14-apelin mice. D: BODIPY (green) and Isolectin (red) staining revealed decreased size of subcutaneous adipocytes (green) in K14-apelin mice after HFD feeding. E: Morphometric analysis of adipocyte size confirmed that adipocytes were smaller in HFD-fed K14-apelin mice. Data are expressed as mean values ± SD. F: Inhibition of lymphatic dye leakage in mesenteric fat of K14-apelin mice after HFD. G: Whole-mount staining of mouse ears using antibodies against LYVE-1 (green) and CD31 (red) revealed that HFD induced enlargement of both lymphatic and blood vessels, whereas no significant difference was found between RD- and HFD-fed K14-apelin mice. Two parallel yellow lines as indicated in (G) show vascular size. Bars indicate 1 mm (C) and 200 μm (D, G). **P < 0.01, *P < 0.05.