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. 2013 May 17;62(6):1923–1931. doi: 10.2337/db12-0977

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© 2013 by the American Diabetes Association.

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FIG. 3. — A and B: RB1 mRNA (A), RB1 total protein (B) (left panel), and RB1 activity (B) (right panel) during time course of differentiation of human preadipocytes isolated from the denoted fat depots of lean (BMI <25 kg/m²) or obese (BMI >30 kg/m²) subjects. Phosphorylated (Phospho) Thr821 RB1 and total RB1 were measured by ELISA. *P < 0.05 in comparison with day 0; **P < 0.005 in comparison with day 0; #P < 0.05 in comparison with day 2; ##P < 0.005 in comparison with day 2; ++P < 0.005 in comparison with day 7. C: Representative immunoblots of ^pThr821RB1 and total RB1 protein during time course of differentiation of human subcutaneous preadipocytes. Immunoblots for FASN as an adipocyte marker and β-actin as an internal control in the same cultures are also shown. D: Bivariate correlation between ELISA and Western blotting measurements of ^pThr821RB1 and total RB1 protein (r > 0.8, P < 0.01). O.D., optical density. (A high-quality color representation of this figure is available in the online issue.)