Skip to main content
. 2013 May 22;8(5):e63759. doi: 10.1371/journal.pone.0063759

Figure 1. ATP was released after infection both in vitro and in vivo.

Figure 1

(A) In vitro analysis of ATP released from BMDMs and bacteria. The ATP levels in the culture medium from wells containing only cells, only bacteria (3×108 CFU/ml) or both were assessed after 0.5, 1 and 2 hour of incubation. The data are presented as the means±SEMs (n = 5). (B) In vivo analysis of ATP release from murine peritoneal cells. Mice received intraperitoneal injections of E.coli 25922 (3×108 CFU per mouse) or S. aureus 25923 (3×108 CFU per mouse). The mice were sacrificed 0.5, 1 or 2 hours after injection. The peritoneal lavage fluid was harvested by injecting 2 ml of PBS into the peritoneum. The ATP concentration in the fluid was then assayed. As a negative control, 150 µl of 0.9% NaCl was intraperitoneally injected, and the peritoneal lavage fluid was harvested 2 hours after NaCl injection. The data are presented as the means±SEMs (n = 10). Values that are significantly different different are indicated by asterisks as follows: **, P<0.01.