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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2013 Jun;72(6):462–471. doi: 10.1097/NEN.0b013e3182933788

Figure 1.

Figure 1

Glia-centered stereology-based study. (a) Diagram represents a typical dissector map resulting from the random sampling of a paraffin section of temporal cortex using the parameters described in the Materials and Methods section. (b) Only glial cells (in this example, glial fibrillary acidic protein [GFAP]-positive astrocytes, numbered, 1, 2, 3) with a DAPI-visible nucleus and whose soma fell within the counting frame and/or touched one of the 2 green sides of the counting frame (but none of the red sides) were considered. (c, d) Next, the presence of a Thioflavin-S-positive dense-core plaque was evaluated in the blue-green channel (c), and, if present, the distance between the glial cells and the closest plaque was measured with the appropriate tool of the CAST stereology software (d). Glial cells were considered close to plaques if their soma was located within 50 μm from the nearest plaque edge and far from them if located beyond this boundary.