Table 2.
Primary Antigen | Vendor/Catalog# | Host | Dilution | Secondary Antibody |
---|---|---|---|---|
GFAP | Sigma (St. Louis, MO) Cat it#G9269 |
Rabbit | 1:1000 | Gt-Cy3 anti-Rb 1:200 |
Iba1 | Wako (Osaka, Japan) Cat #019-19741 |
Rabbit | 1:250 | Gt-Cy3 anti-Rb 1:200 |
CD68 | Dako (Carpinteria, CA) Cat#M0814 |
Mouse | 1:100 | Gt-Cy3 anti-Ms 1:200 |
Abbreviations: Cat, catalogue; GFAP, glial fibrillary acidic protein; Gt, goat; Iba1, ionized calcium-binding adaptor molecule 1. All secondary antibodies were purchased from Jackson ImmunoResearch Labs, West Grove, PA.