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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Nat Chem Biol. 2013 Apr 14;9(6):374–382. doi: 10.1038/nchembio.1230

Figure 7. Effect of the retinoid derivatives in the cellular response against different stressors.

Figure 7

(a) Immunoblot for the indicated proteins in homogenates (Hom) and lysosomes (Lys) isolated from cells untreated (none) or treated for 12 h with 20μM of the indicated compounds. (b) mRNA levels of LAMP-2A in mouse fibroblasts control (Ctr) or knocked down (−) for RARα, and treated with AR7 or paraquat (PQ) as in a (n=4–5). (c) Cellular viability of control (left) or LAMP-2A(−) (right) fibroblasts exposed to 2 mM or 0.5 mM PQ, respectively, and treated with the indicated compounds for 12 h before or after the PQ treatment. (n=3). (d) Viability of mouse fibroblasts transfected with the indicated concentrations of a plasmid encoding α-synuclein and left untreated (none) or treated with 1 mM PQ alone or in the presence of 20μM AR7. (n=3). (e) Immunoblot for α-synuclein in the same cells as d. Top: higher exposure blot to highlight oligomeric (Oligo) species. *nonspecific band. M: monomer; All values are mean±S.E. Differences with cells untreated (*) or treated only with PQ (§) were significant for p<0.001. Full-length blots are shown in Supplementary Figure 21.