Table 1.

Bilayered OPF composite design for four experimental groups tested in this study. Growth factor loading concentration is shown as ng of incorporated growth factor per mL of crosslinked OPF composite hydrogels before swelling.

Experimental groups			Control	Group 1	Group 2	Group 3
Cartilage layer (Top 1mm)	OPF Hydrogel Loading Solution		PBS	PBS	TGF-β3	PBS
	10 mM GMPs	mg per 100 mg OPF Loading solution	11 PBS	11 PBS	11 PBS	11 TGF-β3
	40 mM GMPs	mg per 100 mg OPF Loading solution	11 PBS	11 IGF-1	11 IGF-1	11 IGF-1
	Growth Factor Loading	IGF-1 loading (ng IGF-1/mL composite gel)	0	2000	2000	2000
		TGF-β3 loading (ng TGF-β3/mL composite gel)	0	0	1800	1800
Subchondral layer (Bottom 2 mm)	OPF Hydrogel Loading Solution		PBS	PBS	PBS	PBS
	10 mM GMPs	mg per 100 mg OPF Loading solution	22 PBS	22 PBS	22 PBS	22 PBS
	Growth Factor Loading	IGF-1 loading (ng IGF-1/mL composite gel)	0	0	0	0
		TGF-β3 loading (ng TGF-β3/mL composite gel)	0	0	0	0
Description