Figure 4. Effects of oxidative stress on inflammatory markers in Caco-2/15 cells.

Cells were incubated with Fe/Asc (200 µM/2 mM) and Trolox (0.25 mM) for 6 h at 37°C and/or with 5-AZA (10 µM). The protein expression of Interleukin-6 (IL-6) (A, C) and cyclooxygenase 2 (COX2) (B, D) was determined by western blot as described in Materials and Methods. Results represent the means ± SEM of n = 3 independent experiments. *P<0.05, **P<0.01 vs. controls; ^#P<0.05, ^##P<0.01 vs. Fe/Asc.