Figure 7. SOD2 and GPX2 promoter methylation following Fe/Asc exposition.

Cells were incubated with Fe/Asc (200 µM/2 mM) and Trolox (0.25 mM) for 6 h at 37°C and/or with 5-AZA (10 µM). DNA methylation of SOD2 (A, C) and GPx2 (B, D) was then determined as described in Materials and Methods. Results represent the means ± SEM of n = 3 independent experiments. **P<0.01, ***P<0.001 vs. controls; ^#P<0.05, ^##P<0.01 vs. Fe/Asc.