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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Neuropharmacology. 2012 Jul 20;63(6):1002–1011. doi: 10.1016/j.neuropharm.2012.07.022

Fig. 3.

Fig. 3

Nicotine, acetylcholine and varenicline-induced intracellular calcium response in HEK293 cell lines stably expressing human nicotinic acetylcholine receptor subtypes α3β4, α3β4α5D or α3b4α5N. Stimulation was performed with different concentrations of nicotine, acetylcholine or varenicline in the presence of 10 mM Ca2+. Atropine (200 nM) was included in the assay mix to block muscarinic receptors. Data is normalized to the average maximal response of α3β4 cells. α3β4α5 receptors showed decreased response to agonists. Two-way ANOVAs for the agonist curves: Nicotine, nAChR subtype effect F2,125 = 114.2, p < 0.001; Acetylcholine, nAChR subtype effect F2,104 = 32.46, p < 0.001; Varenicline, nAChR subtype effect F2, 161 = 74.32, p < 0.001. Post-hoc results for α3β4 vs. α3β4α5D: * = p < 0.05, ** = p < 0.01, *** = p < 0.001; for α3β4 vs. α3β4α5N: # = p < 0.05, ## = p < 0.01, ### = p < 0.001; and for α3β4α5D vs. α3β4α5N:$ = p < 0.05,$$ = p < 0.01,$$$ = p < 0.001. Results are given as normalized response values ± SEM.