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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Neuropharmacology. 2012 Jul 20;63(6):1002–1011. doi: 10.1016/j.neuropharm.2012.07.022

Fig. 5.

Fig. 5

Nicotine-induced desensitization in HEK293 cell lines stably expressing human α3β4, α3β4α5D or α3β4α5N nicotinic acetylcholine receptor subtypes. Prior to stimulation, the cells were pretreated for 30 min with different concentrations of nicotine. Thereafter, the cells were stimulated with 1 mM acetylcholine in the presence of 10 mM Ca2+. Atropine (200 nM) was included in the assay mix to block muscarinic receptors. n = 9. Results are given as means of normalized response ± SEM. Data is normalized to the response at 0 μM nicotine within each cell line.