Fig. 7.

Effect of CdCl₂, ryanodine and xestospongin C on acetylcholine-induced calcium response in HEK293 cell lines stably expressing human α3β4, α3β4α5D or α3β4α5N nicotinic acetylcholine receptors. Cells were stimulated with 1 mM acetylcholine in the presence of 10 mM Ca²⁺ and 200 nM atropine. IP₃ receptor blocker, xestospongin C, has a differential effect in cells expressing α3β4 receptors and α3β4α5D/N receptors. More precisely, the IP₃-mediated intracellular calcium release is more pronounced in cells expressing α5* receptors. Two-way ANOVA results for the data presented in this figure: treatment effect: F_{3, 130} = 77.275, p < 0.001; subtype effect: F_2,130 = 23.128, p < 0.001; treatment × subtype interaction: F_{6, 130} = 3.286, p < 0.01. XeC = xestospongin C. ** = p < 0.01, *** = p < 0.001 vs. corresponding treatment in α3β4. n = 8–12. Data is presented as means of percentages of the corresponding control ± SEM. Data is normalized to the response of corresponding control cells to 1 mM acetylcholine within each receptor subtype.