Table 2.
Antigen retrieval and primary antibodies
| Antibody | Origin | Target | Dilution | Antigen retrieval | Source |
|---|---|---|---|---|---|
| AQP-1 | Rabbit (pAB) | Aquaporin-1 | 1:500 | 0 | Sc-20810; Santa Cruz, USA |
| AQP-4 | Rabbit (pAB) | Aquaporin 4 | 1:250 | 0 | Sigma-Aldrich, St. Louis, USA |
| C9neo | Rabbit (pAB) | Complement component C9 | 1:2,000 | P | [39] |
| CD68 | Mouse (mAB) | 110-kD Transmembrane glycoprotein | 1:100 | St (E) | M0814; Dako, Glostrup, Denmark |
| GFAP | Rabbit (pAB) | Glial fibrillary acidic protein | 1:3,000 | St (E) | Z0334; Dako, Glostrup, Denmark |
| Ig | Sheep (pAB) | Human immunoglobulin | 1:200 | P | RPM1003; Amersham Pharmacia Biotech, Buckinghamshire, UK |
| MBP | Rabbit (pAB) | Myelin basic protein | 1:2,500 | 0 | A0623; Dako, Glostrup, Denmark |
| NF | Rabbit (pAB) | Neurofilament medium chain (150 kDa) | 1:2,000 | St (E) | AB1981; Chemicon, Temecula, CA, USA |
| PLP | Mouse (mAB) | Proteolipid protein | 1:1,000 | St (E) | MCA839G; Serotec, Düsseldorf, Germany |
| MAG | Mouse (mAB) | Myelin associated glycoprotein | 1:1,000 | St (E) | AB B11F7 [27] |
| TPPP/p25 | Rabbit (pAB) | Tubulin polymerization promoting protein; Oligodendrocytes | 1:250 | St (E) | [15] |
| MOG | Mouse (mAB) | Myelin associated glycoprotein | 1:1,000 | St (C) | AB 8-18C5 [27] |
pAB polyclonal antibody, mAB monoclonal antibody, St antigen retrieval in steamer, E EDTA buffer, C citrate buffer, P antigen retrieval with protease