Table 1.
Extracellular proteins of C. violaceum identified using MALDI-TOF/TOF MS
| Spot nº | Locus tag | Protein | Cellular localizationa | COG Classb |
|---|---|---|---|---|
| 1, 28, 32, 40, 51, 64, 67, 71 | CV_1097 | Binding protein component of ABC dipeptide transporter | E | E |
| 2 | CV_1709 | Flagellar hook-associated protein FlgK | C | N |
| 3 | CV_3931 | Chitosanase A | E | G |
| 4, 10, 33, 43, 44, 82 | CV_2001 | Collagenase | C | O |
| 5 | CV_3475 | Hypothetical protein CV_3475 | E | S |
| 6, 8, 18, 35, 45, 46, 52, 53, 57, 59, 70, 76, 77, 79 | CV_1440 | Hydrolase transmembrane protein | E | R |
| 7 | CV_4329 | Oligopeptide ABC transporter system, substrate-binding protein | E | E |
| 9, 16 | CV_3739 | Peroxidase | C | P |
| 11, 85 | CV_0349 | Hypothetical protein CV_0349 | C | S |
| 12, 42, 58, 60, 63, 68, 69, 86 | CV_4107 | Hypothetical protein CV_4107 | PSE | S |
| 13, 19, 21 | CV_1710 | Flagellar hook-associated protein FlgL | C | N |
| 14 | CV_4224 | Hypothetical protein CV_4224 | C | S |
| 15, 27, 31, 41 | CV_0350 | Phage sheath protein | C | R |
| 17, 18, 66 | CV_3323 | Carbohydrate-binding protein | E | S |
| 20, 49, 56 | CV_2994 | Flagellar hook-associated protein FliD | C | N |
| 22, 36 | CV_1706 | Flagellar basal body rod protein FlgG | C | N |
| 23, 24, 55, 62, 78 | CV_3977 | Hypothetical protein CV_3977 | C | S |
| 25 | CV_0362 | Thermolabile hemolysin, lecithin-dependent | C | R |
| 26 | CV_0867 | Superoxide dismutase | E | P |
| 29, 30 | CV_1369 | Hypothetical protein CV_1369 | E | S |
| 34 | CV_3276 | Hypothetical protein CV_3276 | E | S |
| 36, 54, 61 | CV_2893 | Hypothetical protein CV_2893 | E | S |
| 37, 83 | CV_4188 | Elongation factor Tu | C | J |
| 38, 39, 47, 49, 73, 75 | CV_3011 | Flagellin D | C | N |
| 48 | CV_0223 | Hypothetical protein CV_0223 | C | H |
| 50 | CV_3506 | Protease precursor | E | R |
| 54 | CV_0408 | Hypothetical protein CV_0408 | C | S |
| 57 | CV_1703 | Flagellar basal body rod modification protein | C | N |
| 65 | CV_4240 | Chitinase | E | G |
| 71 | CV_2390 | Riboflavin synthase subunit beta | C | H |
| 71 | CV_0340 | Tail fiber assembly protein | C | R |
| 72 | CV_0424 | Hypothetical protein CV_0424 | C | R |
| 74 | CV_1415 | γ-glutamyltransferase precursor | E | E |
| 80 | CV_0410 | Bacteriophage tail core protein | C | R |
| 81 | CV_2034 | Peptidoglycan N-acetylmuramoyl hydrolase | E | M |
| 84 | CV_0409 | Bacteriophage tail sheath protein | C | R |
a E extracellular protein, C cytoplasmic protein, PSE potentially surface-exposed protein, according to the SurfG prediction
bClasses of clusters of orthologous groups (COG): (E) amino acid transport and metabolism; (G) carbohydrate transport and metabolism; (H) coenzyme metabolism; (J) translation, ribosomal structure, and biogenesis; (M) cell envelope biogenesis; (N) cell motility and secretion; (O) posttranslational modification, protein turnover, chaperones; (P) inorganic ion transport and metabolism; (R) general function prediction only; (S) function unknown