Fig. 3.

Astrocytes and microglia/macrophages express Sema3A, 3F in MS brain, and oligodendroglial cells express their receptors a Astrocytes (GFAP-labelled) and microglia/macrophages (CD68-labelled) in chronic active lesions express Sema3A. Astrocytes and microglia/macrophages in active and chronic active lesions express Sema3F (active lesion shown) b NP1 and NP2 are expressed in oligodendroglial cells (Nkx2.2-labelled) (chronic active lesions shown). Examples of double-positive cells are labelled with arrows. Pictures are flattened confocal stacks. White boxes indicate location of magnified picture. High background staining is found in this human postmortem tissue and is most obvious in the NP2 photographs where the bright punctate staining is nonspecific. Scale bar 10 μm