Fig. 7.

Manipulation of Sema3A or Sema3F levels in the mouse model in vivo changes OPC migration to the lesion. a Timeline for experiment. b More nkx2.2+ oligodendroglial cells are present around and within lesions treated with rSema3F and less around lesions treated with rSema3A as compared to the unlesioned sides at 2 and 3 weeks after the lesion. More CC1+ oligodendrocytes are present around and within lesions treated with rSema3F and less around lesions treated with rSema3A as compared to the unlesioned side at 3 and 4 weeks after the lesion (mean ratio + SD asterisks on bars indicate value is significantly different as compared to contralateral side (assigned value of 1), symbols above bars indicate significant differences between groups *p < 0.05, ^$ p < 0.001, ^φ p < 0.01, comparing treatments by ANOVA and Tukey’s post test). There is no difference in proliferation (Ki67+ cells) c or apoptosis (cleaved caspase-3+ cells) d within the lesion between the groups at 2 weeks (mean + SD, n > 20 sections from 5 mice per group)