Table 1. Comparison of the multiwell plate-adapted method for measurement of ROM using DEPPD oxidation in the presence of Fe2+ to results obtained with the commercially available FORT kit.
| Within day | Between day | |
|---|---|---|
|
|
|
|
| Method | ||
| FORT kit (Carr Units) (Low) | 273 ± 31 | 270 ± 23 |
| CV (%) | 11 | 8.6 |
| (High) | 495 ± 5 | 485 ± 16 |
| CV (%) | 1.0 | 3.2 |
| Overall CV (%, n=4) | 6.0 | 5.8 |
| Microplate (Carr Units) (Low) | 295 ± 21 | 288 ± 20 |
| CV (%) | 7.1 | 6.8 |
| (High) | 478 ± 22 | 465 ± 19 |
| CV (%) | 5.0 | 4.8 |
| Overall CV (%, n=4) | 6.5 | 5.3 |
| Microplate value as percentage of FORT value (n=4) | 101 ± 4 | 99 ± 0.5 |