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. Author manuscript; available in PMC: 2013 May 23.
Published in final edited form as: ACS Nano. 2012 Feb 1;6(2):1221–1229. doi: 10.1021/nn2038669

Figure 2.

Figure 2

(A) Single-molecule AFM-FRET ultra nanoscopy, the zoomed panel in the left presents schematic diagram of one FRET dye-pair (donor-acceptor: Cy3–Cy5) labeled HPPK molecule tethered between a glass cover-slip surface and a handle (biotin group plus streptavidin), and another biotin group is modified on AFM tip. (B) Single-molecule fluorescence photon counting images of the donor (Cy3, left) and accepter (Cy5, right). Each feature is from a single HPPK enzyme labeled with Cy3–Cy5 FRET dyes.