FIGURE 5.

The long-term memory of conditioned courtship is impaired in heterozygous DmGluRA¹¹² mutant flies. For long-term memory, males were either paired with a non-receptive female for 7 h or sham trained (also referred to as naïve trained) in the absence of a female for the same period. The males from both training classes were then kept in isolation for 4 days then paired with a virgin female, and monitored for courtship activity. The resulting CI after training is compared to the CI obtained for naïve courtship. Mean CIs (± SEM) are plotted; Ns are indicated above each bar for all groups. For levels of significance, ***p < 0.001. Control DmGluRA^2b flies (filled black bars) and control OreR background flies (striped bars) exhibited intact long-term memory as demonstrated by a significant depression of courtship activity in the trained versus the naïve groups. The DmGluRA¹¹² mutant flies (open bars) did not demonstrate a suppression of courtship activity after training and therefore had impaired long-term memory.