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. 2013 May 8;2013:250725. doi: 10.1155/2013/250725

Figure 2.

Figure 2

Inhibition of MIP-2 production (a) and CXCR2 expression (b and c) by kaempferol. Inhibition of MIP-2 production and CXCR2 expression by kaempferol in OVA-challenged mouse lung tissues. Tissue extracts were collected for the measurement of MIP-2 production by using an ELISA kit (a). Immunofluorescence analysis showing inhibition of CXCR2 expression in OVA-challenged mouse lung tissues by kaempferol (b). CXCR2 localization was visualized with a Cy3-conjugated secondary antibody. Nuclear staining was done with DAPI. CXCR2 was identified as red staining and quantified by using an optical microscope system (c). Each photograph was representative of four mice. Magnification: 200-fold. Values in the bar graphs (mean ± SEM) not sharing a common letter are significantly different at P < 0.05.