Figure 3.

Western blot data showing inhibition of eotaxin-1 expression by kaempferol or OxPAPC in LPS- or IL-8-stimulated in BEAS-2B cells (a and b). After culturing cells with 2 μg/mL LPS in the absence and presence of 1–20 μM kaempferol or 20 μg/mL OxPAPC for 8 h, cell extracts were subjected to 15% SDS-PAGE and western blot analysis with a primary antibody against eotaxin-1. β-actin protein was used as an internal control. Inhibition of CCR3 expression and eotaxin-1 secretion by kaempferol in OVA-challenged mouse lung tissues (c and d). Tissue extracts were subjected to western blot analysis with a primary antibody against murine CCR3 (c). The bar graphs (mean ± SEM, n = 3) in the bottom panels represent quantitative results. Eotaxin-1 production was measured in OVA-challenged mouse lung tissues by using an ELISA kit (d). Values in bar graphs not sharing a letter indicate significant difference at P < 0.05.