Figure 4.

Temporal activation of Tyk2 (a) and its blockade by kaempferol in LPS-exposed epithelial cells. BEAS-2B cells were cultured with 2 μg/mL LPS (b) or 10 ng/mL IL-8 (c) in the absence and presence of 1–20 μM kaempferol for 8 h. Total cell protein extracts were subjected to western blot analysis with a primary antibody against phosphorylated Tyk2. β-actin was used as an internal control. The bar graphs (mean ± SEM, n = 4) in the bottom panels represent quantitative densitometric results. Means without a common letter differ, P < 0.05.