Figure 6.

Time course response of STAT3 (a), suppression of STAT1/3 phosphorylation (b), and elevation of SOCS-3 expression (c) by kaempferol in LPS-stimulated BEAS-2B cells. BEAS-2B cells were treated with 1–20 μM kaempferol in the absence and presence of 2 μg/mL LPS for 8 h. For western blot analysis, total cell protein extracts were immunoblotted with a primary antibody against phosphorylated STAT1, phosphorylated STAT3, total STAT3, SOCS-3, or β-actin as an internal control. The bar graphs (mean ± SEM, n = 3) in the bottom panels represent quantitative densitometric results. Means without a common letter differ, P < 0.05.