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. 2013 May 8;2013:810632. doi: 10.1155/2013/810632

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Copyright © 2013 Nabilah Muhammad Nadzri et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Quantitative analysis of ZER-HPβCD inclusion complex mediated apoptosis parameter. Changes in (a) total nuclear intensity, (b) cell permeability, (c) mitochondrial membrane potential, and (d) cytochrome c localization were all measured simultaneously in HepG2 treated cells. Treatment with ZER-HPβCD inclusion complex showing statistically significant cell loss (data not shown), increased total nuclear intensity, increased cell permeability, loss of mitochondrial membrane potential, and cytochrome c release from the mitochondria with good P values. Each experiment was performed at least two times. *Indicates a significant difference (P < 0.05) compared with control.