Figure 4. Endogenous Prf but not IFN-γ in memory CD8⁺ T cells is required for the early control of ECTV LH spread.

A-B) Virus titers at 7 dpi in livers (A) and spleens (B) from B6.D2-D6 mice that received 5×10⁶ N-WT CD8⁺ T cells, or enough MWT, M-IFN-γ^−/− or M-Prf^−/− CD8⁺ T cells to contain ~75,000 K^b-TSYKFESV⁺ cells. Data are representative of three independent experiments. C-J) N-WT, M-WT, M-IFN-γ^−/− or M-Prf^−/− CD8⁺ T cells were labeled with CFSE to identify divided donor cells. 5×10⁶ N-WT CD8⁺ T cells or a number of M-WT, M-IFN-γ^−/− or M-Prf^−/− CD8⁺ T cells that contained ~75,000 K^b-TSYKFESV⁺ cells were transferred into B6.D2-D6-Thy1.1⁺ mice. One day later, the mice were infected with ECTV and at 4 dpi D-LN cells were counted and analyzed by flow cytometry. The indicated parameters were analyzed. Data are represented as a mean ± SEM and are representative of two similar experiments. Representative flow cytometry plots are shown in (E). Also see Figure S3 for Cidofovir treated mice and confocal microscopy, respectively.