Skip to main content
. Author manuscript; available in PMC: 2014 May 28.
Published in final edited form as: J Control Release. 2013 Feb 16;168(1):41–49. doi: 10.1016/j.jconrel.2013.02.004

Figure 3.

Figure 3

Association of NC with 9% surface mannoside (Formulation 2.4). (a) Time and temperature dependence of cellular association with J774E cells as a function of time at 4°C (□) and 37°C (■). Extent of association was quantified by cell lysis and solubilization of the hydrophobic dye. (b-d) Representative confocal images of fixed J774E cells, corresponding to the following conditions: (b) cells not exposed to NCs (c) cells exposed to NCs for 3 h at 4°C, and (d) cells exposed to NCs for 3 h at 37°C. The DAPI nuclear stain is shown in red and EtTP5 fluorescence is shown in green.