. Author manuscript; available in PMC: 2013 May 23.

Published in final edited form as: Methods Mol Biol. 2013;980:157–173. doi: 10.1007/978-1-62703-287-2_8

Table 2.

Reaction contents for TaqMan-based Q-RT PCR reactions

Reagent	Stock concentration	Volume (μL)	Final concentration (nM)
TaqMan Universal PCR Master Mix	2×	10.0
Forward primer	20 μM	0.9	900
Reverse primer	20 μM	0.9	900
Hydrolysis probe	5 μM	1.0	250
cDNA	N/A	4.0
Nuclease-free water	N/A	3.2
Total		20