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Reaction conditions for TaqMan-based Q-RT PCR analysis
| Name | Number of cycles | Step | Target temperature (°C) | Hold time | Ramp rate (°C/s) | Notes |
|---|---|---|---|---|---|---|
| Preincubation | 1 | 1 | 95 | 10 min | 4.4 | |
| Amplification | 45 | 1 | 95 | 10 s | 4.4 | |
| 2 | 60 | 30 s | 2.2 | Fluorescence acquisition | ||
| 3 | 72 | 1 s | 4.4 | |||
| Cool | 1 | 1 | 40 | 30 s | 2.2 |
