Figure 2. Hyperosmolality-induced decreases in force that were aggravated by low K⁺ conditions and prevented by bumetanide.

(A) Soleus from R669H^+/m mice (n = 6) was more susceptible to reductions in force due to hyperosmolar challenge as compared to WT (n = 6). A concomitant reduction in K⁺ levels from 4.75 to 2 mM resulted in a complete loss of force for R669H^+/m soleus. (B) Pretreatment with bumetanide (BMT) (75 μM) prevented decreases in force.