Table 2.
Cdc42 knockdown decreased the tumourigenic capacity of BLBC cells in a c-Cbl dependent manner
| Number of cells injected | ||||
|---|---|---|---|---|
| 1 × 106 | 1 × 105 | 1 × 104 | 1 × 103 | |
| MDA-MB 231 | ||||
| Ctrl shRNA | 6/6 | 5/5 | 7/7 | 4/5 |
| Cdc42 shRNA | 8/8 | 5/5 | 3/8* | 1/5* |
| c-CblshRNA | ||||
| Ctrl shRNA | – | 5/5 | 5/6 | 5/5 |
| Cdc42 shRNA | – | 5/5 | 5/8 | 3/5 |
| HCC1954 | ||||
| Ctrl shRNA | – | 5/5 | 3/5 | – |
| Cdc42 shRNA | – | 4/5 | 0/5* | – |
| HCC70 | ||||
| Ctrl shRNA | – | – | 2/3 | – |
| Cdc42 shRNA | – | – | 0/2 | – |
Animals were transplanted with the number of cells indicated, and tumour progression was monitored by whole mouse imaging every 20 days till three months post transplantation.
*
p < 0.05 in comparison of tumour development between animals xenografted with cells expressing scrambled or Cdc42 shRNAs.