Figure 3.

SRSF1 and SRSF9 are required for Wnt-induced β-catenin accumulation.

A. SRSF1 and SRSF9 are required for Wnt1-stimulated reporter expression. HEK293T cells were transfected with Wnt-responsive TOPFLASH reporter, Wnt1 plasmids, human SRSF1-siRNA-2 and SRSF9-siRNA-1 as indicated. Mouse SRSF1 (mSRSF1) or Xenopus SRSF9 (xSRSF9) was able to rescue the β-catenin reporter activation.

B,C. RKO cells were transfected with siRNAs as indicated and 48 h later, the cells were treated with Wnt3a protein (100 ng/ml) for 1 h and total cell lysates were harvested and proceeded by SDS–PAGE and Western blotting. The relative expression of β-catenin against Tubulin were quantified with three independent experiments.

D,E. HCT116 cells were transfected with siRNAs as indicated and 48 h later, total cell lysates were prepared and proceeded by SDS–PAGE and Western blotting with indicated antibodies. The relative expression of β-catenin against Tubulin were quantified with three independent experiments.