Figure 1.

Anti-Vel Western blot of non-reduced RBC membrane extracts identifies a 32 kDa band present only in Vel+ individuals.

RBC membrane extracts prepared from two Vel+ (lanes 1 and 3), one Vel+^w (lane 2), and two Vel− (lanes 4 and 5) subjects were resolved by non-reducing SDS–PAGE, and probed with an anti-Vel (top panels). A long exposure time was required to detect the 32 kDa reactive band in the Vel+^w subject (top right panel), who later appeared to be heterozygous for the 17 nt deletion in SMIM1. The same blot was reprobed with anti-7.2b (bottom panels).