R-848 stimulates production of SPMs and their precursors from deuterium-labelled DHA in monocytes and macrophages.
A. LC-MS-MS chromatograms of d5-7-HDHA, d5-14-HDHA and d5-17-HDHA produced by untreated (solid blue line) and R-848-treated (dashed red line) mouse macrophages.
B,C. Increases in mouse macrophage d5-RvD1 and d5-PD1: quantitation (left) and LC-MS-MS identification (right) by R-848-treated mouse macrophages. Results are expressed as mean ± SEM of n = 4 for d5-RvD1 and n = 3 for d5-PD1.
D. Increase in human monocyte d5-PD1: quantitation (left) and LC-MS–MS identification (right) by R848-treated human monocytes. Results are representative of n = 3. Proresolving lipid mediators were identified according to published criteria (Yang et al, 2011). *p < 0.05; Xp < 0.05 compared to 0; #p < 0.05 compared to 1.