Figure 5.

R-848 up-regulates the expression of 5-lipoxygenase and 12/15-lipoxygenase enzymes.

Mouse macrophages were stimulated for 1 h with 5 µg/ml R-848, 1 µg/ml LPS or vehicle-treated control (CTL) and analyzed by real-time PCR.

A. Alox-5 and Alox-15 mRNA levels in peritoneal macrophages expressed as mean levels ± SEM relative to GAPDH (n = 6 from two independent experiments).

B. Alox-5 and Alox-15 mRNA levels in bone marrow-derived macrophages expressed as mean levels ± SEM relative to GAPDH (n = 6–10 from two independent experiments). *p < 0.05, **p < 0.01, ns: non-significant compared to CTL.